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首页>试剂>细胞、模式生物>细胞株>3T3-L1 (小鼠前脂肪细胞)
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| Organism | Tissue | Morphology | Culture Properties |
| Mus musculus (Mouse) | Embryo | Fibroblast | Adherent |
| General Information | |
| Cell Line Name | 3T3-L1 (Mouse Preadipocytes Cells) |
| Synonyms | 3T3-L1 (小鼠前脂肪细胞) |
| Organism | Mus musculus (Mouse) |
| Tissue | Embryo |
| Cell Type | Fibroblast |
| Morphology | Fibroblast |
| Disease | － |
| Strain | － |
| Biosafety Level* | 1 |
| Age at Sampling | Embryo |
| Gender | Male |
| Genetics | A substrain of 3T3-Swiss albino cell line |
| Ethnicity | － |
| Applications | These cells are a suitable transfection host |
| Category | Spontaneously immortalized cell line |
| Characteristics | |
| Karyotype | － |
| Virus Susceptibility | － |
| Derivation | L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. |
| Clinical Data | － |
| Antigen Expression | － |
| Receptor Expression | Insulin, expressed |
| Oncogene | － |
| Genes Expressed | Triglycerides |
| Gene expression databases | － |
| Metastasis | － |
| Tumorigenic | － |
| Effects | － |
| Comments | The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090]. Tested and found negative for ectromelia virus (mousepox). This line is also designated as ATCC CCL-92.1. ATCC CL-173 was deposited in 1974 without passage number information from the depositor |
| Culture Method | |
| Doubling Time | － |
| Methods for Passages | Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
| Medium | DMEM (high glucose)+10% FBS |
| Special Remarks | － |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | － |
| Growth Condition | 95% air+ 5% CO2, 37℃ |
| Freeze medium | DMEM (high glucose)+20% FBS+10% DMSO， |
