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水平拉针仪
2021-06-17 10:56:23
一、功能原理
在生物学实验中，通常要将电极很精确的插入细胞中，电极通常是玻璃管拉制出来的，微电极
拉制仪是将玻璃管拉制成电极的常用工具，其基本原理是将玻璃管放置在加热丝中，然后控制
加热丝的温度，以达到将玻璃管烧断，在给玻璃管加热的同时，在玻璃管的两端给一定拉力，
将玻璃管拉制成各种需要的电极，供实验需要。美国 Sutter 公司的 P-97 程控水平微电极拉制
仪常用于各种玻璃，石英等微电极的拉制制作，作为在实验中常用的玻璃，石英等微电极，在
电生理，细胞注射的实验中广泛被应用，而美国 Sutter 公司的 P-97 程控水平微电极拉制仪被
确定为电生理，细胞注射的实验室的标准配置仪器。
二、主要功能和用途：
制备各种玻璃电极，在电生理，细胞注射的实验中广泛被应用于拉制微电极的工具
三、主要性能指标
1. 湿度控制室
2. 气压可控
3. 可存贮多达 100 个程序
4. 写保护锁和日期封印
5. 每次拉制都产生两个对称的电极
6. 为胞内记录和膜片钳电极设计的例子程序可以修改，可对特殊需求进行编程
7. Ramp 检测--当使用新灯丝或新玻璃时能对加热设置进行自检验
8. 为灯丝和拉制螺线管提供恒流电源
9. 为拉制膜片钳用电极设计的循环周期拉制
10. 真空荧光显示
11. 自带的供气装置有湿度控制和过滤系统
12. 能拉制稳定、可靠的尖端小于 0.1 微米的电极
13. 能控制空气释放的时间和压力
14. 内部存储器检验
15. 优化的速度检测回路有最大的敏感度和重复性
16. 质量控制：电镜检测电极尖端变化小于 0.1 微米，一般大约为 0.06 微米
Developed through years of experience with the Flaming/Brown style micropipette pullers and infused with leading edge technology, Sutter is proud to introduce the P-1000 micropipette puller. The most obvious new feature is the color touchscreen display that provides an intuitive and full-featured interface.
The extensive library of programs found in the popular Sutter Cookbook has been incorporated into the P-1000 puller and is available to the user via the touchscreen display. You need only specify the glass, filament, and type of pipette you require and a suitable program will be identified and available for installation. This takes the guesswork out of pipette pulling and simplifies programming.
The Pre-heat mode actively heats and controls the jaw temperature and assures that the jaws have reached a specific temperature before the glass is pulled. This can increase the stability of the program from pull to pull. Copy and paste functions assure that programs can be easily written and the line repeat mode simplifies multi-line programming. A safe heat mode is an additional feature that helps the user avoid using heat settings that might damage or burn out the filament. When the safe heat mode is turned on, the puller will “check” the installed heat and alert the user when a given heat value is too low or too high in relation to the ramp value. User notes can be added to each program for annotating important information.
New features for the P-1000 include: diagnostic testing of all puller components, built-in error detection of air pressure loss or filament burnout, easy access to ramp test, measurement of jaw temperature, and access to previous pull results with the heat on times for each cycle of the program. In addition, help topics are pre-loaded to assist with on-site troubleshooting and the built-in glossary includes text, pictures, and diagrams explaining the terms used in micropipette fabrication. A rotary dial is offered as an alternative to the keypad for numerical entry.
COMMON APPLICATIONS P-1000
Patch pipettes
Sharp electrodes
Pronuclear injection
Zebrafish injection
Insect egg injection
Aspiration pipettes
- FEATURES
Color touch-screen display
Safe heat mode to protect and extend filament life
Pipette Cookbook program directory
Pre-heat mode improves stability
Line repeat mode simplifies multi-line programming
Help topics and error detection
Glossary with micropipette and puller terminology
Copy & Paste function for writing new programs
Jaw temperature sensor helps define ideal pulling conditions
Record of last two pull results
Ramp test more easily accessed and can now be stored and referenced within each program. Helps to establish program heat settings and protect filament.
Two symmetrical pipettes with each pull
Self-contained air supply with filtration system and humidity control chamber
Memory storage for up to 100 programs
Two cooling modes: time and delay
购买链接：
2、 水平拉针仪P-1000 (biotop100.com)
